Part:BBa_K4811032:Design
PT7-TMS(PFOA3)-TT7
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 84
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As of 2023, the TMS is a novel BioBrick, and not much is known about the specifics of TMS to RBS binding, as well as how an aptamer insertion into the D-loop will alter this binding, both in presence and absence of the ligand.
Multiple versions of a PFOA-sensitive TMS were attempted, in this instance, the PFOA-aptamer was inserted without the stem, from J. Park et. al. was included into the D-loop.
Source
The part was ordered as a gBlock from IDT.
The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.
The PFOA-sensitive aptamer was developed via SELEX , see the publication "Novel ssDNA aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water", Junyoung Park, Kyung-Ae Yang, Yongju Choi, Jong Kwon Choe, Environment International, 2022, 158 107000.